The Effect of Nucleic Acids on the Development of Certain Enzymic Activities in Disrupted Staphylococcal Cells by E. F. Gale and Joan P. Folkes
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چکیده
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منابع مشابه
The assimilation of amino acids by bacteria. 24. Inhibitors of incorporation of glycine in disrupted staphylococcal cells.
In previous papers of this series (Gale & Folkes, 1955a, b) a description has been given of a preparation of disrupted staphylococcal cells which can be used to study the dependence on nucleic acid of protein-synthesis and amino acid-incorporation reactions. Removal of nucleic acid from the preparation results in marked decrease in the incorporation of certain amino acids; the incorporation can...
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Gale & Folkes (1953a, b) showed that there is an inorease in the nucleic acid content of washed suspensions of Staphylococcus aureus (Micrococcu4s pyogenes var. aureus) when these are incubated in the presence of glucose, purines, pyrimidines and a mixture of all those amino acids necessary for protein synthesis. Omission of an essential amino acid resulted in cessation of nucleic acid synthesi...
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From Table 2 it will also be seen that the diabetogenic dose of alloxan in normal animals, as found in this laboratory, ranges between 130 and 140 mg./kg. and the lethal dose is observed to be in the neighbourhood of 225 mg./kg. It is interesting to note that the diabetogenic and lethal doses of alloxan in acetoacetate-treated animals, having blood GSH in the neighbourhood of 3 mg./100 ml., are...
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In previous papers of this series, experimental conditions have been described which will enable the following processes to be studied in washed suspensions of Staphylococcus aureus (Micrococcus pyogenes var. aureus): internal accumulation of free glutamic acid (Gale, 1947 a, b), extracellular accumulation of peptides containing glutamic acid (Gale & Van Halteren, 1951), accumulation of combine...
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